Figure EV5. Additional examples of curious behaviors and potential manifestations.

1. For RTN2, the noise of transcriptional output shows a non‐monotonic scaling with Msn2 amplitude for 10 min Msn2 single‐pulse duration, but a monotonically decreasing relationship for 50 min Msn2 duration. The average transcriptional output increases monotonically with Msn2 amplitude for both durations.
2. For SIP18 mutant D6, time active is largely independent of pulse length for 25% Msn2 amplitude but increases with pulse length for higher Msn2 amplitudes. The transcriptional output averaged over all cells (bottom panel, blue) increases with pulse length and Msn2 amplitude. However, the average transcriptional output of only responding cells is very similar for both Msn2 amplitudes. The examples shown in (A, B) illustrate further interesting behaviors and potential manifestations, but we emphasize that more analysis will be required to validate the robustness of these results.