Table 1.
List of assessments to be carried out at various time points
| Mothers | Baseline |
| Physical health | Anthropometry: weight, height; body composition: bioimpedance, skinfolds (subset), DXA (subset), stable isotope (subset); blood pressure: digital sphygmomanometer |
| Biospecimen | Blood sample: plasma, serum, DNA, RNA (subset) |
| Questionnaires | Diet: food frequency questionnaire (FFQ), 24 hours food recall, diet diversity and food security questionnaires; physical activity: Global Physical Activity Questionnaire (GPAQ); Sleep: Pittsburgh Sleep Quality Index (PSQI); General Self Efficacy Scale (GSES); Generalised Anxiety Disorder-7 (GAD-7); Patient Health Questionnaire−9 (PHQ-9); Adverse Childhood Experience (ACE) questionnaire sociodemography; socioeconomic status (Standard of Living Index; SLI); medical/ treatment/drug history; smoking and alcohol history |
| Pregnancy | |
| Physical health | Anthropometry: weight; body composition: bioimpedance, stable isotope (subset); blood pressure: digital sphygmomanometer |
| Biospecimen | Blood: oral glucose tolerance test (OGTT), plasma, serum, DNA, RNA (subset); urine; saliva (subset) |
| Questionnaires | FFQ, 24 hours food recall, diet diversity and food security; GPAQ; PSQI; GSES; GAD-7; Edinburgh Postnatal Depression Scale (EPDS); Social Provision Scale; Breast-feeding Self-efficacy Scale; Perceived Stress Scale Sociodemography; socioeconomic status (SLI); medical/ treatment/drug history; smoking and alcohol history |
| Delivery | |
| Weight; stool/rectal swab (subset); delivery details | |
| Serial postnatal follow-up (up to 60 months) | |
| Physical health | Anthropometry: weight; body composition: DXA (subset), stable isotope (subset); blood pressure: digital sphygmomanometer; OGTT; blood samples |
| Biospecimen | Blood: plasma, serum, DNA, RNA (subset), OGTT |
| Questionnaires | FFQ; 24 hours food recall, diet diversity and food security; GPAQ; PSQI; GSES; GAD-7; EPDS; PHQ-9; Social Provision Scale; Breast feeding Self-efficacy Scale; Perceived Stress Scale; Parenting Stress Index; Sociodemography; Socioeconomic status (SLI); medical/ treatment/drug history; smoking and alcohol history |
| Fathers | Index pregnancy |
| Anthropometry: weight, height; body composition: bioimpedance, DXA (subset); blood pressure: digital sphygmomanometer blood: plasma, serum, DNA, RNA (subset), saliva (subset) FFQ; 24 hours food recall, diet diversity and food security; GPAQ; PSQI; PHQ-9 sociodemography, socioeconomic status (SLI); medical/ treatment/drug history; smoking and alcohol history |
|
| Child | Birth |
| Anthropometry: weight, length, head/arm/abdomen/chest circumference, skinfolds DXA, cord blood; placenta; umbilical cord; meconium |
|
| Birth to 12 months | |
| Anthropometry: weight, length, circumferences; body composition: DXA, stable isotope (subset) feeding history; vaccination history; medical/treatment/drug history stool |
|
| Serial postnatal follow-up (major follow-up at 24 and 60 months) | |
| Anthropometry: weight, length/height, circumferences; body composition: DXA, blood pressure; blood samples (heel prick at 24 months and venous sample at 60 months): plasma, serum, DNA, RNA, glycaemia feeding history; sleep questionnaire; ages and stages questionnaire; neurocognitive developmental tools: Developmental Assessment Scale for Indian Infants (DASII) at 24 months; modified Kaufmann Battery at 60 months; executive function assessment tools; physical activity (questionnaire and accelerometry); screen time questionnaire; general health assessment; vaccination history; medical/treatment/drug history |