FIGURE 4.

FSS‐mediated activation of TGFβ and BMP R‐Smads require their corresponding ligand. A, Representative western analysis of control or ligand‐treated cells grown in culture dishes revealing independent regulation of pSmad bands. B, Anti‐Flag co‐immunoprecipitation (left panels) or western (Input, right panels) of lysates from control or Flag‐Smad transfected OCY454 cells, followed by corresponding western analysis with antibodies against Flag or pSmad2/3. C, D, Representative western analysis of cells pretreated (60 minutes) with vehicle, 1d11 TGFβ‐blocking antibody (C), or the BMP ligand antagonist Noggin (D) followed by stimulation (30 minutes) with TGFβ (5 ng/mL), BMP4 (50 ng/mL), or FSS (0.1 Pa) with Smads labeled as identified in B