Table 1. . Algorithm used for real-time data to flag atypical amplification curves for removal in real-time dPCR experiments.

	False positive to be removed	Standard (which will be removed)
1	Fluorescent signal increases but then decreases before completion of thermocycling	FAM20/FAM15 <0.95 or FAM25/FAM20 <0.95 or FAM30/FAM25 <0.95 or FAM35/FAM30 <0.95
2	Fluorescent signal comes up too late	(FAM39-FAM35)/(FAM39-FAM15) >0.3
3	Fluorescent signal starts high in early cycles or remains relatively flat during thermocycling	FAM20 >MEAN(FAM20) + 6*SD(FAM20) or (FAM35-FAM25)/25 <0.1
4	Fluorescent signal shows minimal increase between cycle 25 and cycle 35	(FAM30-FAM25)/(FAM39-FAM15) <0.33
5	Fluorescent signal shows too much increase between cycle 10 and cycle 15	0.92 <FAM15/FAM10 <1.08

SD: Standard deviation.