Table 4. . Retesting of ‘aged’ 1–2 × limit of detection contrived clinical samples by real-time dPCR and end point dPCR (May batch).
| Sample | Read by real-time dPCR | Read by end point dPCR | ||||
|---|---|---|---|---|---|---|
| FAM+ | Judgment | Positive rate | FAM+ | Judgment | Positive rate | |
| S20 | 2 | + | 86.36% | 2 | - | 54.55% |
| S21 | 0 | - | 0 | - | ||
| S22 | 2 | + | 6 | + | ||
| S23 | 1 | + | 7 | + | ||
| S24 | 2 | + | 2 | - | ||
| S25 | 1 | + | 1 | - | ||
| S26 | 2 | + | 7 | + | ||
| S27 | 2 | + | 6 | + | ||
| S28 | 1 | + | 1 | - | ||
| S29 | 2 | + | 5 | + | ||
| S30 | 1 | + | 1 | - | ||
| S31 | 1 | + | 5 | + | ||
| S32 | 1 | + | 1 | - | ||
| S33 | 6 | + | 7 | + | ||
| S34 | 3 | + | 3 | + | ||
| S35 | 3 | + | 3 | + | ||
| S36 | 0 | - | 2 | - | ||
| S37 | 0 | - | 4 | + | ||
| S38 | 2 | + | 1 | - | ||
| S39 | 2 | + | 5 | + | ||
| S40 | 1 | + | 1 | - | ||
| S41 | 3 | + | 7 | + | ||
The ‘aged’ samples were a set of 1–2 × limit of detection clinical samples (similar in composition to those in Table 3) that had been aged for 2 months at -80°C.