FIGURE 2.

Biopanning of the Fyn SH2 variant library to enrich the pTyr binders. (a) Phage ELISA of the selected phage from each biopanning round and the naïve library binding to the phosphotyrosine peptide (EPQpYEEIPIYL) and the nonphosphorylated counterpart, respectively. R0 refers to the naïve library. R1 to R4 stand for phage panning cycle round 1 to round 4. (b) The sequences of ELISA confirmed single SH2 variant clones (n = 22) were aligned and the sequences logo of the eight positions was generated. The sequence of the eight positions of wild type Fyn SH2 was also shown at the top for comparison