FIGURE 8.

Isothermal titration calorimetry of H364A and E81A PAI‐1. Cu(II) titration of active H364A using ITC is shown in panel (a); latent H364A PAI‐1 in panel (b); active E81A PAI‐1 in panel (c); and latent E81A PAI‐1 in panel (d). ITC data are baseline corrected in NITPIC software and fit to either a single‐site model (active and latent H364A PAI‐1) or two‐site binding model (active and latent E81A PAI‐1) using SEDPHAT. The data and fit are represented in GUSSI by plotting heat of injection (kcal/mol) against the copper/PAI‐1 molar ratio