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. Author manuscript; available in PMC: 2021 Feb 17.
Published in final edited form as: Cell Rep. 2021 Feb 2;34(5):108714. doi: 10.1016/j.celrep.2021.108714

Figure 4. Voltage-activated K+ channels constrain the subthreshold oscillation in RTN neurons.

Figure 4.

(A) Subthreshold oscillations (0.5 μM TTX, at −40 mV with DC injection), before and after TEA (10 mM).

(B) Phase-plane plot of averaged oscillation in control (blue) and TEA (orange).

(C), Integrated power and mean frequency of subthreshold oscillations before and after TEA (mean ± SEM, n = 7). RM ANOVA with Sidak’s post hoc: F1,6 = 35.11, p < 0.001 for TEA effect on power, ****p < 0.0001; F1,6 = 0.5135, p = 0.5006 for TEA effect on frequency.

(D) Subthreshold oscillations (0.5 μM TTX, at −45 mV and −40 mV with DC injection) under control conditions and in the presence of the KCNQ channel inhibitor ML252 (10 μM).

(E) Effect of ML252 on integrated power and mean frequency of subthreshold oscillations at the indicated membrane potentials (mean ± SEM, n = 9). RM ANOVA with Sidak’s post hoc: F1,8 = 17.08, p = 0.0033 for ML252 effect on power, *p < 0.05; F2,16 = 4.265, p = 0.0328, for frequency x membrane potential test; +, p = 0.0231.

(F and G) I-V plots of oscillation-waveform-induced currents in cells recorded with a K+-based pipette solution reveal outward currents sensitive to TEA (F) and ML252 (G).

(H) TEA and ML252 significantly increased peak oscillation-waveform-evoked inward current (control versus TEA: p = 0.007, n = 7; control versus ML252: p = 0.02, n = 6; by paired t test). Box and whiskers plots as in Figure 1.