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. Author manuscript; available in PMC: 2021 Feb 17.
Published in final edited form as: Cell Rep. 2021 Jan 26;34(4):108682. doi: 10.1016/j.celrep.2020.108682

Figure 5. H5N1 vaccine-elicited mAbs target discrete regions on HA and share footprints with pAbs.

Figure 5.

(A–C) Side views of negative stain EM reconstructions or 2D class averages show that subjects 4 and 43 day 7 mAbs target the stem domain (A) while day 28 mAbs target the vestigial esterase domain (B) and the RBS (C) of recombinant H5 HA (A/Indonesia/5/2005).

(D) Comparison of subjects 4 and 43 mAb and pAb immune complexes. Due to limited particle representation, orange, yellow, and pink pAbs from subject 43 are predicted placements.

(E) Cross-reactivity of example mAbs from subjects 4 and 43 to recombinant HAs from A/Indonesia/5/2005 (H5N1), A/California/04/2009 (H1N1), A/Singapore/1/1957 (H2N2), and A/Singapore/INFIMH-16–0019/2016 (H3N2). mAbs are shown in solid colors: 4.7.1C01, pale blue; 43.7.1A06, light blue; 43.7.1B02, teal; 43.7.1B04, dark blue; 4.28.1F03, purple; 4.28.1H09, lavender; 43.28.1A02, plum; 43.28.1A08, maroon; 43.28.1H01, brick; and 43.28.1E01, brown. pAbs from day 28 are shown in colored mesh: stem specificities, blue and green; RBS-proximal and lateral patch specificities, red, orange, and yellow; vestigial esterase and mid-lateral head specificities, pink.

See also Figure S5.