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. Author manuscript; available in PMC: 2021 Feb 17.
Published in final edited form as: Cell Rep. 2021 Jan 26;34(4):108682. doi: 10.1016/j.celrep.2020.108682

Figure 6. High-resolution cryoEM defines broadly reactive epitopes of H5N1 vaccine-elicited pAbs.

Figure 6.

(A) CryoEM-mapped epitopes targeted by pAbs from subject 4 at day 28. RBS epitope, red; lateral patch epitope, yellow; vestigial esterase epitope, pink; VH1–69 epitope, blue.

(B) Each epitope specificity marked together on a single trimer.

(C and D) CryoEM map of stem immune complex from subject 4 at day 28 with ribbon diagrams for H5 HA (A/Indonesia/5/2005, gray; PDB: 4K62) and mAb 1C01 from subject 4 day 7 docked into the EM density. Full-length side view (C) and zoomed in view of the epitope-paratope interaction (D). VH, blue; VL, green; CDRH2, dark blue; CDRH3, purple; HA residues H18 and W21, red.

See also Figure S6.