Table 2. Lysis solutions tested.

No.	Lysis solution
1	Milli-Q water
2	50 mM BNB (pH 11.0)
3	50 mM PB (pH 8.5)
4	50 mM BNB (pH 11) with SDS* and 50 U ProK
5	50 mM PB with SDS* and 50U ProK
6	50 mM BNB with SDS*, 50U ProK and dithiothreitol*
7	50 mM PB with SDS*, 50U ProK, and dithiothreitol*
8	50 mM BNB with SDS*, 50U ProK, and mercaptoethanol*
9	50 mM PB with SDS*, 50U ProK, and mercaptoethanol*

BNB: Borate-NaOH buffer, PB: Phosphate buffer, ProK: Proteinase K.

*The concentration was adjusted to 1% of the premix volume.