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Schematic diagrams show two protocols with different staining sequences (A). Multiplex IHC and single color images were obtained using these two protocols from human tonsil (FFPE) tissue serial sections. H&E images show the morphology (B, C). Singleplex IHC in adjacent slides verified individual biomarker stains in mIHC (B, C). The mIHC stain followed Protocol 1 in (B) and Protocol 2 in (C).
