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| Antibody | Clone | Species | Titration | Fluorophore | Vendor |
|---|---|---|---|---|---|
| PD-L1 | E1L3N | Rabbit | 1:1600 | Opal690/Opal520 | Cell Signaling |
| CD163 | MRQ-26 | Mouse | 1:4 | Opal620 | Roche/Ventana |
| CD8 | SP16 | Rabbit | 1:400 | Opal650 | Spring Biosciences |
| CD3 | SP7 | Rabbit | 1:600 | Opal540 | Genetex |
| Foxp3 | 236A/E7 | Mouse | 1:400 | Opal520/Opal690 | Abcam |
| Cytokeratin | AE1/AE3 | Mouse | 1:3000 | Opal570 | Dako |
