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. 2021 Feb 4;15(2):e0009059. doi: 10.1371/journal.pntd.0009059

Fig 1. Expression of inhibitory receptors in the CD4+ T cell population in chronic Chagas disease patients, including indeterminate patients and patients with cardiac alterations.

Fig 1

(A) The frequencies of CD4+ T cells expressing 2B4, CD160, CTLA-4, PD-1 or TIM-3 in 38 chronic Chagas disease patients (cChD), including 25 indeterminate patients (IND) and 13 patients with cardiac manifestations (CCC), were calculated for each sample and compared to those frequencies in 12 healthy donors (HD). (B) The expression levels of the inhibitory receptors under study in CD4+ T cells were measured according to the value of the mean fluorescence intensity (MFI) and represented in a box-plot for each marker in each study group (HD, cChD, IND and CCC). The analysis was performed by flow cytometry and data calculated using the FlowJo 9.3.2 software. Cell cultures were in vitro stimulated with T. cruzi soluble antigens (TcSA) for 10 h in the presence of CD28 and CD49d co-stimulators. The range of values represented by the box and the whiskers includes the values from the minimum to the maximum. Statistical analyzes were performed using the Mann-Whitney U test. The p values are represented by asterisks as follows: p<0.05 (*), p<0.01 (**), p<0.001 (***) and p<0.0001 (****). (C) Example of representative plot of the frequency of CD4+ T cells that express CTLA-4 in a healthy donor (HD), an indeterminate (IND) and a patient with cardiac symptomatology (CCC).