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. 2021 Feb 4;15(2):e0009059. doi: 10.1371/journal.pntd.0009059

Fig 4. Impact of benznidazole treatment on the level of the coexpression of inhibitory receptors in CD4+ T cells, as evaluated in chronic Chagas disease patients.

Fig 4

The inhibitory receptors evaluated in the coexpression study were 2B4, CD160, CTLA-4, PD-1 and TIM-3. The box-plot graphs show the percentages of CD4+ T cells coexpressing 2, 3, 4 or 5 inhibitory receptors, as evaluated before and after treatment with benznidazole, in indeterminate patients (IND, n = 25) and cardiac patients (CCC, n = 15). The tested time points were T0, pretreatment; T1, 9–12 months after treatment administration; and T2, after 24–48 months. The coexpression studies of the five inhibitory receptors under study were carried out using a Boolean gates strategy using FlowJo 9.3.2 software. The range of values represented by the box and the whiskers includes the values from the minimum to the maximum. Statistical analyzes were performed using the Wilcoxon test. The p values are represented by asterisks as follows: p<0.05 (*) and p<0.01 (**).