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. 2021 Feb 4;17(2):e1008859. doi: 10.1371/journal.pgen.1008859

Fig 2. Neuronal expression of dGba1b rescues protein aggregation in Gba1b mutants.

Fig 2

(A-D) Using the neuronal driver elav-GAL4, wildtype dGba1b was expressed in Gba1b mutants and wildtype revertant controls. Homogenates were prepared from fly heads and bodies using 1% Triton X-100 lysis buffer. Western blot analysis was performed on the Triton X-100 soluble fractions using antibodies to Ref(2)P and Actin and on the insoluble proteins using antibodies to ubiquitin (Ub) and Actin. Representative images and quantification of Ref(2)P in (A) bodies (one-way ANOVA: F(3,7) = 153.581, p < 0.001) and (B) heads (F(3,6) = 542.043, p < 0.001) and Ub in (C) bodies (F(3,8) = 6.099, p = 0.018) and (D) heads (F(3,8) = 16.878, p < 0.001) of control and Gba1b mutants with and without neuronal expression of dGba1b are shown. Results are normalized to Actin and control. At least 3 independent experiments were performed. Error bars represent SEM. *p < 0.05 by Student t-test. (E) Kaplan-Meier survival curves of control, Gba1b mutants, and Gba1b mutants and controls expressing WT dGba1b using the elav-GAL4 driver.