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. 2021 Feb 17;7(8):eabe1969. doi: 10.1126/sciadv.abe1969

Fig. 4. Matrix composition and growth factor signaling jointly regulate 3D invasion and matrix remodeling by MCF10AT premalignant breast cancer cells.

Fig. 4

(A) Schematic of the experiment. (B) Confocal microscopy of spheroid morphology after 6 days of culture in either col/rBM (left) or rBM (right) in the presence or absence of TGFβ. F-actin fibers (green false color) were stained with TRITC-phalloidin. Vimentin (cyan false color) was stained by immunofluorescence as an EMT marker. Nuclei (magenta false color) were visualized using an endogenously expressed H2B-GFP fusion protein. Scale bars, 50 μm. (C) DLSμR measurements of the impact of TGFβ treatment on particle MSD over the course of tissue culture for cells embedded in either col/rBM (left) or rBM (right). Curves are the geometric mean among independent biological replicates (n = 3 to 5). Error bands represent 68% confidence intervals of the geometric mean.