Skip to main content
. 2021 Feb 17;7(8):eabe1969. doi: 10.1126/sciadv.abe1969

Fig. 5. TGFβ promotes active force fluctuations, collagen fiber remodeling, and matrix stiffening by MCF10AT breast cancer cells in a col/rBM ECM.

Fig. 5

(A) Analysis of the thermal contribution to the frequency-dependent force fluctuation spectrum after 6 days in col/rBM. (B) Effect of TGFβ on the active force fluctuation spectrum in col/rBM after 6 days. (C) Confocal microscopy of collagen fiber organization after 6 days of culture with cells in col/rBM. Collagen I (white false color) was stained by immunofluorescence. F-actin fibers (green false color) were stained with TRITC-phalloidin. Nuclei (magenta false color) were visualized using an endogenously expressed H2B-GFP protein. Scale bar, 50 μm. (D) Effect of TGFβ on the frequency-dependent shear modulus G* after 6 days of culture in col/rBM. In (A), (B), and (D), curves represent the arithmetic mean among biological replicates, and bands represent 68% confidence intervals of the means (n = 3 for −TGFβ and n = 5 for +TGFβ).