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. 2021 Feb 17;7(8):eabf1738. doi: 10.1126/sciadv.abf1738

Table 1. BLI kinetics for immobilized sACE22-IgG1 binding to coronavirus RBDs.

CoV strain* WT sACE22-IgG1 sACE22.v2.4-IgG1
kon (M1 s−1) koff (s−1) KD (nM) χ2‡ kon (M−1 s−1) koff (s−1) KD (nM) χ2
LYRa11 8.7 × 105 7.9 × 10−2 91 0.12 1.4 × 106 2.5 × 10−3 1.8 0.10
Rs7327 6.4 × 105 4.0 × 10−2 63 0.25 9.8 × 105 1.8 × 10−3 1.9 0.11
Rs4231 3.2 × 105 2.2 × 10−2 69 0.04 4.5 × 105 1.6 × 10−3 3.5 0.10
Rs4084 2.9 × 105 2.5 × 10−2 85 0.24 4.8 × 105 1.5 × 10−3 3.1 0.10
RsSHC014 8.8 × 105 2.6 × 10−2 29 0.20 1.6 × 106 2.0 × 10−3 1.3 0.29
SARS-1 6.6 × 103 1.2 × 10−4 58 0.03 3.0 × 103 5.6 × 10−6 2.1 0.03
SARS-2 1.4 × 106 8.1 × 10−3 16 0.25 6.6 × 105 2.8 × 10−4 0.4 0.09
SARS-2 (Y449K) 2.0 × 106 9.0 × 10−2 46 0.67 4.3 × 106 4.0 × 10−3 0.9 0.71
SARS-2 (N501W) 2.4 × 106 5.4 × 10−3 2.3 0.43 3.3 × 106 2.8 × 10−4 0.1 0.23
SARS-2 (N501Y) 2.2 × 106 1.8 × 10−3 0.8 0.15 3.6 × 105 1.1 × 10−4 <0.1 0.24

*Purified RBDs at five to seven concentrations were used as the soluble analytes.

†IgG1 Fc–fused sACE22 was immobilized to anti-human IgG Fc capture biosensors.

‡χ2 values represent the goodness of curve fitting. Acceptable values were considered to be less than 3.