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. 2021 Feb 17;7(8):eabe2597. doi: 10.1126/sciadv.abe2597

Fig. 2. ISWI knockdown results in circadian arrhythmicity, memory deficits, and social dysfunction.

Fig. 2

(A) Representative actogram traces from individual flies for each genotype. (B) Comparison of rhythm strength in the setting of ISWI knockdown (red) and in genetic controls (black and gray), as measured by FFT (n = 58, 55, and 70 from left to right). (C) Proportion of strongly rhythmic, moderately rhythmic, weakly rhythmic, and arrhythmic flies in the setting of ISWI knockdown and in genetic controls. (D) Experimental design of PER. (E) Quantification of proboscis extensions in ISWI knockdown (red, n = 39) compared to genetic controls (black, n = 30; gray, n = 47) (two-way ANOVA with post hoc multiple comparison test; asterisks denote significance of ISWI knockdown condition compared to both genetic controls in post hoc testing). (F) MB structure with FasII staining in controls (left) and ISWI knockdown (right). Scale bar, 50 μm. (G) Male courtship indices (left) and copulation success (right) in the setting of ISWI knockdown compared to genetic controls (n = 60, 49, and 81 from left to right). (H) Social space index in ISWI knockdown and genetic controls (n ≥ 3 replicates per genotype, 40 flies per replicate per genotype) (see Materials and Methods for details on calculation of index).