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. 2021 Feb 17;7(8):eabe2597. doi: 10.1126/sciadv.abe2597

Fig. 3. ISWI knockdown through mid-third instar leads to adult sleep disruptions.

Fig. 3

(A) Schematic of TARGET system. (B) Quantification of total sleep duration in the setting of pre- (left) or post-eclosion (right) ISWI knockdown (blue) compared to genetic controls (black and grays) exposed to the same temperature shifts across development or constitutive knockdown (red) (for pre-eclosion: n = 82, 46, 57, 47, and 58 from left to right; for post-eclosion: n = 82, 46, 52, 28, and 40). Quantification of total sleep duration in the setting of ISWI knockdown through (C) first instar (n = 82, 31, 43, 33, and 36 from left to right) and (D) second instar (n = 82, 69, 46, 57, and 50 from left to right). ns, not significant. (E) Total sleep duration and (F) night sleep duration, sleep bout length, and sleep bout number (left to right) in the setting of ISWI knockdown through mid-third instar (n = 82, 84, 68, 55, and 58 from left to right). (G) Summary of ISWI knockdown periods and resulting effects on total sleep duration.