Fig. 7. The human ISWI homologs SMARCA1 and SMARCA5 differentially rescue sleep and memory.

(A) Representative sleep traces with ISWI knockdown (red) compared to SMARCA1^WT (blue) and SMARCA5^WT (green) expression in the setting of ISWI knockdown or overexpression alone (black) with (B) quantification of total sleep and (C) activity index across experimental and control groups (n = 104, 48, 29, 102, and 55 from left to right). (D) Representative images of FasII immunostaining of adult brains with pan-neuronal ISWI knockdown (left), SMARCA1^WT rescue (middle), and SMARCA5^WT rescue (right; scale bar, 50 μm) and (E) quantification of MB lobe volume across groups (n = 5, 21, 16, and 14 from left to right). (F) Quantification of proboscis extensions in ISWI knockdown (red, n = 30) compared to genetic control (black, n = 37), SMARCA1^WT rescue (blue, n = 29), and SMARCA5^WT rescue (n = 26) (two-way ANOVA with post hoc multiple comparison test). (G) Representative sleep traces of pan-neuronal ISWI knockdown (red) compared to rescue with SMARCA5^WT (green) or SMARCA5^del268-319 (purple) and overexpression of WT or mutant constructs alone, with (H) quantification of total sleep duration (left) and night sleep duration (right) (n = 104, 102, 55, 41, and 31 from left to right). For graphs in this figure, letters represent statistically similar groups.