Fig. 4. Transcriptional profiling identified MAML2 as a conserved inhibitor of Wg/Wnt signaling.

(A and B) Venn diagrams depicting the genes that are activated (A) or repressed (B) after Dox treatment (13.5 hours) of pTRE-SOX9^WT and pTRE-SOX9^EFD-AAA cells. (C and D) Volcano plots summarizing the differently expressed genes pTRE-SOX9^WT (A) and pTRE-SOX9^EFD-AAA (B) cells treated with Dox for 13.5 hours. Location of MAML2 indicated by red dot in both plots. (E) Schematic representation of human MAMLs and Drosophila Mam proteins. The location of the polyglutamines (>12 sequential Qs), conserved acidic domains, and the N-terminal CSL/N^intra binding domains are indicated. (F to K) Micrographs of adult eyes of Drosophila containing P[GMR-wg] (F and I), P[GMR-arm*] (G and J), or P[GMR-hid] (H and K) transgenes without (F and G) or with (I to K) a P[EP] line expressing Mam. (L) Quantification of the adult eye phenotypes using ImageJ (n = 10 eyes per genotype). Each bar represents the means of biological triplicates ± SD. See Materials and Methods for details. ***P < 0.001; **P < 0.005. Photo credit: Ken M. Cadigan, University of Michigan.