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. Author manuscript; available in PMC: 2021 Feb 17.
Published in final edited form as: Cell Rep. 2020 Feb 25;30(8):2644–2654.e3. doi: 10.1016/j.celrep.2020.01.110

Figure 2: Single-molecule degradation by ClpAP and ClpAP.

Figure 2:

(A) Optical-trap setup with ClpA attached to one bead via ClpPplatform and a multi-domain substrate attached to the smaller bead via linkage of the Halo domain to DNA. (B) Representative single-molecule degradation traces for ClpAP and ClpAP in the N-to-C direction (left) or C-to-N direction (right). The Halo domains are shown in gray. Rectangular and elliptical boxes mark pauses and pre-unfolding dwell times, respectively. Traces were recorded at forces between 8 and18 pN and were decimated to 300 Hz. ClpAP traces are from Olivares et al (Olivares et al., 2017; Olivares et al., 2014) and are shown for comparison.