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. Author manuscript; available in PMC: 2021 Feb 17.
Published in final edited form as: Cell Rep. 2020 Feb 25;30(8):2644–2654.e3. doi: 10.1016/j.celrep.2020.01.110

Figure 4: N-to-C and C-to-N translocation.

Figure 4:

(A) Average N-to-C translocation velocities and pause-free velocities for ClpAP and ClpAP. The inset shows the average number of pauses for one titin domain. Values are the mean ±1 SEM (For ClpAP, n = 86 events). (B) Average C-to-N translocation velocities and pause-free velocities. Other details are the same as in panel (A). (For ClpAP, n = 44 events) (C) Percentage of events with at least one pause during N-to-C or C-to-N translocation of one titin domain for ClpAP and ClpAP. (D) Average step dwell time(s) at each amino acid during N-to-C translocation of a titin domain by ClpAP (red) or ClpAP (black). The average pre-step dwell time at each amino acid was calculated, and a moving average over a 10-residues window is plotted. The shaded region illustrates ±1 SEM of the moving average at each amino acid. (E) Same analysis as in panel (D) except for C-to-N translocation with ClpAP data colored blue.