Table 1:

Unfolding and Translocation parameters with the substrate N-to-C V15P and C-to-N V13P

			N-to-C V15P
	Pre-unfolding dwell time (s)	Intermediate lifetime (ms)	Average translocation velocity (nm/s)	Pause-free translocation velocity (nm/s)	Step dwell time (s)
ClpAP	18.6–19.3 (68–70%)a 0.9–1.1 (30–32%) n = 58	24.8–26.3a n = 76	0.97 ± 0.06b n = 86	2.8 ± 0.1b n = 86	0.83 ± 0.002b (80%), 7.4 ± 0.03b (20%) n = 798
ClpAP	0.77–0.85a	10.56–10.62a n = 109	2.4 ± 0.1b,c	2.9 ± 0.2b n = 109	0.55 b,c
			C-to-N V13P
ClpAP	14.7–17.1 (73–78%)a 1.1–1.4 (24–26%) n = 39	18.4–19.2a n = 50	2.1 ± 0.1b n = 58	2.9 ± 0.2b n = 58	0.6 ± 0.01b (92%), 7.4 ± 4.4b (8%) n = 442
ClpAP	3.0–4.2 (59–63%)a 0.24–0.28 (39–41%)	17.3–17.6a n = 36	3.0 ± 0.1b	3.5 ± 0.2b n = 35	0.4b, d (90%), 2 b,d (10%)

^aThe range indicates 95% CI of the normal distribution of fitting parameters from a bootstrap analysis of the experimental data

^bErrors are ± 1 SEM

^cFrom Olivares et al (Olivares et al., 2017)

^dFrom Olivares et al (Olivares et al., 2014)