KEY RESOURCES TABLE.

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
E.coli BL21DE3	New England Biolabs	C2527H
E.coli JK10 ((clpP::cat, Δlon, slyD::kan, λDE3))	Kenniston et al, 2003	N/A
Chemicals, Peptides, and Recombinant Proteins
ssrA partial peptide for N-terminal substrate maleoyl-β-Ala-NYALAA-coo−	Synthesized using Fmoc technique and an Apex 396 solid-phase peptide synthesizer	N/A
Glass binding peptide CGGRSGRRRSHHHRL	Synthesized using Fmoc technique and an Apex 396 solid-phase peptide synthesizer	N/A
HaloTag Succinimidyl Ester ligand O4	Promega	P6751
Sulfosuccinimidyl 4-[N-maleimidomethyl] cyclohexane-1-carboxylate	Fischer Sci Co	PI22622
Glucose Oxidase, Recombinant	EMD Millipore	345386
Catalase from Bovine liver	Sigma Aldrich	C30
Bovine serum albumin	New England Biolabs	B9000S
BirA, Biotin ligase	Sigma-Aldrich	SRP0417
Oligonucleotides
M13MP18 double stranded DNA	Bayou Biolabs	P-105
5BiosG/AATCCGCTTTGCTTCTGAC	IDTDNA, USA	N/A
5AmMC6/TTGAAATACCGACCGTGTG	IDTDNA, USA	N/A
Recombinant DNA
pFN18A plasmid containing Halo- (V13P)4-ssrA	Cordova et al, 2014	N/A
pFN18A plasmid containing Cys- (V15P)4-Halo	Olivares et al, 2017	N/A
pET23b plasmid containing ClpAE286Q/ΔC9	This study	N/A
pET23b plasmid containing ClpAΔC9	Olivares et al, 2014	N/A
pKS028 plasmid containing ClpPM5A	Olivares et al, 2014	N/A
pKS028 plasmid containing ClpP	Olivares et al, 2014	N/A
Software and Algorithms
Step finding algorithm	Kerssemakers et al, 2006	N/A
Other
1.25 micron streptavidin coated polystyrene beads	Spherotech	SVP-10–5 1%w/v, 1.25
1.09 micron streptavidin coated polystyrene beads	Spherotech	SVP-10–5 1%w/v, 1.09