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Expected results for Quality Controls.
| Quality control | Control of | Expected N1 result | Expected N2 result | Expected RP result |
|---|---|---|---|---|
| Positive control (CTR POS) | RT-qPCR efficiency & primers/probe integrity | Cq < 35 | Cq < 35 | Cq < 35 |
| Human Specimen Control (CTR HSC) | RNA isolation & potential contamination | Cq > 35 | Cq > 35 | Cq < 35 |
| Negative Control (CTR NEG) | RT-qPCR mix or reagent contamination | Cq > 35 | Cq > 35 | Cq > 35 |
