Figure 2.

Expression and purification of PPRV H protein. (a) Analysis of the expression of H protein in the supernatant of transfected cells by Western blot using α-HA antibody (lane 1), together with an equivalent sample from untransfected cells (lane 2) or cells transfected with MV-H head domain plasmid (lane 3). (b) SDS-PAGE and Coomassie staining of the fractions eluted from the α-HA-Sepharose column. The red box highlights the presence of proteins with the predicted size in the fraction 2 of the eluate. (c) Size exclusion chromatogram of the previously obtained fraction 2 showing the presence of a single oligomeric species. Expected elution volumes of known protein calibration standards are indicated