Figure 2. ORF68 and homologs are zinc finger-containing proteins.

(a–c) The Zn²⁺ ion within the three zinc finger motifs is shown as a blue-gray sphere, while coordinating cysteines and histidines are shown in sticks. (d, e) Western blot of whole cell lysate (33 μg) from HEK293T cells that were transfected with plasmids encoding wild-type or mutant variants of ORF68 (e) or UL32 (f). Vinculin serves as a loading control. (f) ORF68.stop iSLK cells were lentivirally transcomplemented with empty vector or with plasmids encoding wild-type or C52A ORF68. Progeny virion production by these cell lines was assayed by supernatant transfer and flow cytometry of target cells. (g) Western blot of transcomplemented ORF68.stop iSLK cells used in (e).