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. 2021 Feb 4;10:e60454. doi: 10.7554/eLife.60454

Figure 5. Integer coupling is maintained over physiological range.

Figure 5.

(a) Extracellular recordings of pdn show bursting of LG and PD at 7°C (blue) and 23°C (orange). Black bars indicate burst period of LG and mean burst period of PD. LG (b) and DG (c) burst period as a function of mean PD period. In (b–c), gray lines have integer slopes and are not fits. (d) Cumulative probability distributions (c.d.f.s) of significand of ratio of gastric to pyloric period for LG (magenta) and DG (green) for all temperatures. Shading indicates confidence interval estimates from bootstrapping the data. Dashed diagonal line indicates a uniform distribution, and the dotted line indicates perfect integer coupling. (e) Area between significand c.d.f.s and diagonal (a measure of how integer-coupled the circuits are) as a function of temperature for all preparations. Gray lines are computed from shuffled data. LG and PD were significantly integer coupled between 7°C and 21°C (p < 10–5, D > 0.24, two-sample Kolmogorov-Smirnoff test), while DG and PD did not exhibit integer coupling. (f) Ratio of gastric to pyloric periods as a function of temperature. Apparent banding along the temperature axis is a consequence of the experimental protocol, where discrete temperature steps were used (Materials and methods). Ratios of LG to PD cycle period were n across temperature (p = 0.88, Spearman test).