Figure 7.

KHSRP involves in HOTTIP facilitated OS progression in vitro. (a) Cell viability of OS cells transfected with si-HOTTIP and pcDNA3.1-KHSRP was detected using CCK-8. (b) Cell cycle of OS cells treated with si-HOTTIP and pcDNA3.1-KHSRP was analyzed by flow cytometry analysis. (c) Colony formation assay was used to estimate colony-formation ability of cells transfected as described in (A). (d) Flow cytometry was allowed to test the effects of HOTTIP/KHSRP axis on cell apoptosis. (e-f) Cell invasion and migration were measured by transwell and wound healing assays. (g) Protein expression of β-catenin, cyclin D1 and c-myc were examined by western blot in OS cells treated with si-HOTTIP and pcDNA3.1-KHSRP. All data were represented by 3 independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001