Figure 2.

Construction, identification and expression of PFK-1 shRNA recombinant plasmids. (a) Schematic diagram of the recombinant pYr-1.1-hU6-EGFP vector. (b) RT-PCR analysis identified the PFK-1 shRNA recombinant plasmids after digestion with restriction enzyme XhoI. The level of sh-PFK1-507 mRNA expression was higher compared with other RNA interference fragment. (c) RT-PCR analysis of PFK1 mRNA expression. The β-actin mRNA and protein expression served as controls for sample loading. (d) Immunofluorescence microscopy images (left: bright field, right: green fluorescence EGFP) of intracellular trafficking of different PFK1 in CNE2 cells at optimal ratios