Figure 4.

Effect of sh-PFK1-507 on apoptosis and proliferation of CNE2 cells. (a) Flow cytometry analysis of cell apoptosis. Cells were incubated in culture medium for 24 h after transfected with sh-PFK1-507. (b) The mean± SD (Standard deviation) percentage of apoptosis cells measured from Flow cytometry. Compared with control cells, the apoptotic rate of sh-PFK1-507 cells significantly increased (39.01 ± 5.08%; p < 0.001). (c) Detection of cell viability via MTT assay. Proliferation of CNE2 cells stably transfected with sh-PFK1-507, vector or untransfected CNE2 cells were evaluated. The highest inhibitory rate of sh-PFK1-507 transfected cells was 33.69 ± 3.47% (p < 0.05). (d–e) Apoptosis proteins (active caspase-3, active caspase-9, and Bax) were increased, and antiapoptosis (Bcl-2 and PCNA) were reduced in CNE2 cells transfected with sh-PFK1-507. All experiments were performed three times with consistent and repeatable results