Figure 2.

The function of circ-FOXM1 in NSCLC cells and tumor growth in vivo. (a-i) H1581 and A549 cells were transfected with sh-NC or sh-circ-FOXM1. (a) Circ-FOXM1 expression was determined by RT-qPCR. (b) CCK-8 was used to assess cell viability. (c) Flow cytometry was employed to analyze cell apoptosis rate. (d) The protein expression of cleaved-caspase-3 and cleaved PARP was detected by western blot. (e) Western blot assay was performed to detect the levels of LC3-I, LC3-II, and P62. (f) Cell migratory ability was measured by transwell assay. (g and h) Tumor volume and weight were calculated in sh-NC mice and sh-circ-FOXM1 mice. There were six mice in each group. (i) The mice body weight was calculated. Student’s t-test was performed for statistical analysis. *P < 0.05