Figure 4.

The role of miR-149-5p in circ-FOXM1-regulated NSCLC progression. (a) MiR-149-5p expression was detected in H1581 and A549 cells transfected with miR-NC or miR-149-5p; Student’s t-test was performed for statistical analysis. (b-g) H1581 and A549 cells were transfected with miR-NC, miR-149-5p, miR-149-5p + Vector, or miR-149-5p + circ-FOXM1. (b) Cell viability was assessed by CCK-8; ANOVA followed via Tukey’s test was performed for statistical analysis. (c) Cell apoptosis was analyzed using flow cytometry; ANOVA followed via Tukey’s test was performed for statistical analysis. (d) Transwell assay was employed to measure cell migratory ability; ANOVA followed via Tukey’s test was performed for statistical analysis. (e) The relative level of cleaved-caspase-3 and cleaved-PARP was detected; ANOVA followed via Tukey’s test was performed for statistical analysis. (f and g) The levels of LC3-I, LC3-II, and P62 were investigated by western blot assay; ANOVA followed via Tukey’s test was performed for statistical analysis. *P < 0.05