Figure 6.

The role of ATG5 in miR-149-5p-regulated NSCLC cell progression. (a-e) H1581 and A549 cells were transfected with sh-NC, sh-ATG5, sh-ATG5 + Anti-miR-NC, or sh-ATG5 + Anti-miR-149-5p. (a) Cell viability was measured by CCK-8 assay. (b) Flow cytometry was used to analyze cell apoptosis rate. (c) Transwell assay was employed to determine cell migration. (d) The relative level of cleaved-caspase-3 and cleaved-PARP was determined. (e) Western blot assay was carried out to detect the levels of LC3-I, LC3-II, and P62. ANOVA followed via Tukey’s test was performed for statistical analysis. *P < 0.05