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. 2021 Feb 9;13(1):1826746. doi: 10.1080/19490976.2020.1826746

Figure 3.

Figure 3.

Administration of B. adolescentis ATCC15703 induces gut barrier reinforcement

(a) Representative pictures in Alcian blue & PAS stained (scale bars, 100 µm; arrows, mucus layer). (b) Western blot analysis of the tight junction related proteins like occludin and claudin in the colon tissue of the negative control mice and PBS or B. adolescentis ATCC15703 gavage mice with DSS. (c) The mRNA expression of the occludin gene in colon tissue. (d,e) The mRNA expression of gene muc2 and muc3. Each experiment was repeated triplicate. Data are expressed as mean ± SD, n = 6–9. *p < .05, **p < .01 (ANOVA test or Kruskal-Wallis test). NC, negative control; DSS, dextran sulfate sodium; PBS, phosphate buffered saline; B.a, Bifidobacterium adolescentis.