Figure 3.

Adipoq-Cre transgenic mouse passenger gene expression analysis. Transcriptional changes were assessed between 8-week-old male Adipoq-Cre^± mice (black bars) and Cre-negative littermate controls (grey bars) in the genes (a) Kng2, (b) Kng1, (c) Eif4a2, (d) Rfc4, (e) Adipoq and (f) Tbx18, revealing increased gene expression in several passenger genes and a consistent decrease in Tbx18 gene expression due to transgene insertion in the native Tbx18 locus. BAT, brown adipose tissue; iWAT, inguinal white adipose tissue; pgWAT, perigonadal white adipose tissue. CPM, normalized counts per million. All values are mean ± SD (n = 4/group); ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001