Figure 6.

Anti-human EGFR-IgG1 or IgG4 subclass effects on the ADCP and ADCC of tumor cell lines by human U937 monocytes. U937 in vitro mediated mainly ADCP (a) and lower levels of ADCC (b) of EGFR differently expressing A431, HT-29 and CaCo2 tumor cell lines (c). The percentage of tumor dead cells was evaluated by flow cytometric analysis following the gating strategy represented on Repository figure 2. Black bars: A431 cells; white bars: HT-29 cells; gray bars: CaCo2 cells. The results from three independent experiments were combined for statistical analysis. One-way ANOVA with Tukey post-hoc test was performed. ** P < .01