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. 2020 Jul 12;16(2):177–185. doi: 10.1080/15592294.2020.1790802

Table 5.

Primers used for methylation analysis

Primer Sequence
CysLTR1 Forward GAAAGAGGAAGAAGAATTTGTATATTTG
CysLTR1 Reverse – biotin AACTTATCCCCAAACTCCTAAAAATT
CysLTR1 cg0081399 Sequencing TTGGAGTTTGTTTTTGAG
CysLTR1 cg26848126 Sequencing ATAGGGTATTAAGATATTGTGTA
ALOX5 cg1559007 Forward GTGGGTATTTTGGTGGGTAAG
ALOX5 cg1559007 Reverse – biotin TCTACCTCTCCCTCCCTATAC
ALOX5 cg1559007 Sequencing AAGGTTTTTTTTTTATTTTAAGATT
ALOX5 cg2430202529 Forward GATGTTTAGAGTTTATTTTTGGTGTTATAT
ALOX5 cg2430202529 Reverse – biotin CTCCTTCTAAAACCTTTTCTATTTCC
ALOX5 cg2430202529 Sequencing AGGTAGGGTAGATATTGATTTT
GPR99 cg24663970 Forward – biotin GGTTGAGGTTTAGAGAAGAGAA
GPR99 cg24663970 Reverse CCAAAAATTTCACCACTCTCC
GPR99 cg24663970 Sequencing ATATCCAATTACAACCCT
LTC4 S Forward ATTGTTTAGATTATAGGGTTTGAGTG
LTC4 S Reverse – biotin TCCCCATCCAAACTATTCCTAACA
LTC4 S cg14674859 Sequencing AGTGTGGGGAGGGTA
LTC4 S cg1631890 Sequencing TGTGGGGTTTAGTGT
LTC4 S cg26683398 Sequencing GGTATTGGGAAGGGT
GPR17 cg0561273 Forward GGTTTTTTTGGAGGAAGTGG
GPR17 cg0561273 Reverse – biotin CAACCCCCAAAACTCTTTAACA
GPR17 cg0561273 Sequencing TGGTTTGAGTTGAGTTT