Figure 1.

Identification of BMSCs-EVs. (a), representative image of primary BMSCs (× 200); (b,c), Oil red staining and Alizarin red staining were performed to validate the lipogenesis and osteogenic differentiation ability of BMSCs (× 200); (d), BMSCs surface markers (CD44, CD54, CD34 and CD90) were detected by flow cytometry; (e), transmission electron microscope of EVs at 5000 demonstrating homogenous, cup‐shaped vesicles with size in 80 nm. Scale bar represents 200 nm in both panels; (f), Nanoparticle tracking analysis of BMSCs-EVs; (g), western blot analysis assessed expression of EV markers in BMSCs