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TGF-β agonist counteracts the protective effect of EVs on NPCs. (a,b), Immunofluorescence of col II and Aggrecan (×200); (c), SA-βgal staining was utilized for NPC senescence measurement (×200); (d), PI/Annexin labeled cells were performed to measure apoptosis index by flow cytometry. Three independent experiments were performed. Data are expressed as mean ± s.d. One-way ANOVA and Tukey’s multiple comparisons test were used to determine statistical significance
