Figure 3.

Susceptibility of C. albicans strains deleted in BGL2 and ECM33 to Calcofluor white (CFW). (A) C. albicans SC5314 (wild-type), bgl2∆/∆ (BGL2M4), ecm33∆/∆ (ECM33M4), and bgl2∆/∆,ecm33∆/∆ (BEM4) were serially diluted and spotted onto YPD agar plates and YPD agar plates containing 12.5 μg/mL CFW and incubated at 30°C for 24 h; (B) C. albicans SC5314 (wild-type), and restored strains bgl2Δ/BGL2 (BGL2MK2), ecm33Δ/ECM33 (ECM33MK2), and bgl2Δ/BGL2,ecm33Δ/ECM33 (BEMK2) were serially diluted and spotted onto YPD agar plates and YPD agar plates containing 12.5 μg/mL CFW and incubated at 30°C for 24 h: (C) C. albicans SC5314 (wild-type), als1∆/∆ (ALS1M4), als1∆/∆,bgl2∆/∆ (ABM4), als1∆/∆,ecm33∆/∆ (AEM4), and als1∆/∆, bgl2∆/∆, ecm33∆/∆ (ABEM4) were serially diluted and spotted onto YPD agar plates and YPD agar plates containing 12.5 μg/mL CFW and incubated at 30°C for 24 h