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Expression of C. albicans ALS genes in wild-type and mutant strains. C. albicans strains were cultured in YPD medium and then total RNA was isolated and subjected to qRT-PCR. Gene expression was normalized against the C. albicans ACT1 gene and is reported for each ALS gene relative to expression in wild-type strain SC5314. All experiments consisted of triplicate measurements (technical replicates) and experiments were carried out three times (biological replicates) (± SD). (*p < 0.05)
