Figure 2.

(a) Low-intensity ultrasound (LIUS) upregulates 21 out of 1376 (1.5%) innatomic genes and downregulates 17 out of 1376 (1.2%) innatomic genes in mouse preosteoblast cells (GSE45487), suggesting that LIUS increases innatomic gene expressions slightly more than decreasing them in mouse preosteoblast cells (see supplemental Table 2 for the detailed gene list). LIUS upregulated 21 innatomic genes that were significantly involved in one signaling pathway in mouse preosteoblasts, which is inflammation-driven cancer metastasis signaling. (b) LIUS downregulated 17 innatomic genes that were not significantly involved in any signaling pathway in mouse preosteoblast cells, suggesting that LIUS inhibits innatome in preosteoblasts in multipathways in a nonsignificant manner.