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. 2021 Feb 10;2021:8889313. doi: 10.1155/2021/8889313

Figure 9.

Figure 9

Administration of sRAGE suppresses inflammation in lung tissues of CLP-induced septic mice. Mice were subjected to CLP or sham surgery. Recombinant sRAGE protein (200 μg/kg) was intratracheally injected immediately before the onset of sepsis, with an equivalent volume of saline intratracheally injected in other groups. Levels of (a) IL-6 mRNA, (b) IL-6 protein, (c) MCP-1 mRNA, and (d) MCP-1 protein in lung tissues. (e, f) Lung tissues were stained with fluorophore-labeled antibodies against macrophage marker F4/80 (red). Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Original magnification, ×200. Scale bars correspond to 50 μm (e). (f) The proportion of F4/80-positive cells in total cells (%). Data are presented as the mean ± SEM (n = 7). ∗p < 0.05 and ∗∗p < 0.01 vs. the sham group; #p < 0.05 and ##p < 0.01 vs. the CLP group.