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. 2021 Jan 28;6(1):ysab004. doi: 10.1093/synbio/ysab004

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© The Author(s) 2021. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — A Biofoundry workflow for the automated screening of microbial interactions. A mutant library of Pseudomonas sp. Ps652ΔHCN was created by automated selection of 2880 colonies (A). These were used in an automated bioassay with S. coelicolor M145 (B) and image analyzed after 5 days of incubation (C). Candidate strains were validated in growth assays and against the target pathogen, S. scabies, in a licensed laboratory (D) before the insertion site was identified by PCR and sequencing (E).