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. 2021 Feb 4;12:622201. doi: 10.3389/fpls.2021.622201

FIGURE 4.

FIGURE 4

ABA affects AtS40.4 expression and protein localization. (A) qRT-PCR analysis of AtS40.4 mRNA level in seedlings. The 5-day-old seedlings grown on 0.5 × MS medium were cultured with free and 10 μM ABA treatment for 1 and 6 h. GAPDH was used as an internal control. Data are means ± SEs of three biological replicates. Double asterisks indicate significant differences (Student’s t-test; **P < 0.01). (B) GUS activity of transgenic proAtS40.4:GUS seedlings with or without ABA treatment. The 3-day-old seedlings grown on 0.5 × MS medium were treated with 10 μM ABA or mock treatment solution for 6 h before GUS staining. This experiment was repeated three times with similar results. Scale bars, 200 μm. (C) Subcellular localization of AtS40.4 in root tip cells of ats40.4 seedlings expressing proAtS40.4:AtS40.4-GFP. Seedling roots were immersed into 100 μM ABA for 0, 2, or 4 h before observation or were transferred onto 24% PEG-6000 (−0.75 Mpa) medium for further growth 1 day as moderate drought stress. Nuclei were labeled with DAPI (shown in a pseudo color representation). Scale bars, 20 μm. (D) Numbers of AtS40.4-GFP particles in a root tip cell of seedlings with free or 100 μM ABA, and 24% PEG-6000 applications. Data are means ± SEs based on more than 10 roots each treatment. Double asterisks denote statistical differences with Student’s t-test at P < 0.01.