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. 2021 Feb 4;12:622201. doi: 10.3389/fpls.2021.622201

FIGURE 6.

FIGURE 6

AtS40.4 functions upstream of ABI4 in ABA signaling. (A) Seed germination and seedling growth of wild type, ats40.4-1 and abi4 single mutants, and ats40.4-1 abi4 double mutant. Seeds were geminated and grown on 0.5 × MS medium containing 0, and 1.0 μM ABA. (B) Seed germination ratios of wild type, ats40.4-1, abi4 and ats40.4-1 abi4 mutants. Seeds were grown on 0.5 × MS medium containing 0 (left) and 1.0 μM ABA (right). The asterisks indicate a significant difference of mutants from the wild-type Arabidopsis (Student’s t-test; *P < 0.05; **P < 0.01). (C,D) Root elongation of seedlings of wild-type, ats40.4-1, abi4, and ats40.4-1 abi4 mutants. The 3-day-old seedlings grown on 0.5 × MS medium were transferred onto the 0, 5, and 10 μM ABA medium for further growth 7 days. (C). Values are means ± SE of three independent biological replicates. Significant differences (D) are denoted with an asterisk (P < 0.05) or double asterisks (P < 0.01).